Polymers Enables Reagent-Free Covalent

Atmospheric Pressure Plasma Jet Treatment of Polymers Enables Reagent-Free Covalent Attachment of Biomolecules for Bioprinting

3D bioprinting, where cells, hydrogels and structural polymers can be printed layer by layer into complex designs, holds great promise for advances in medicine and the biomedical sciences. In principle, this technique enables the creation of highly patient specific disease models and biomedical implants.
However, an ability to tailor surface biocompatibility and interfacial bonding between printed components, such as polymers and hydrogels, is currently lacking. Here we demonstrate that an atmospheric pressure plasma jet (APPJ) can locally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step process at desired locations.
Polyethylene and poly--caprolactone (PCL) were used as example polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removal by rigorous sodium dodecyl sulfate (SDS) washing. The immobilized protein and hydrogel layers were analyzed using Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS).
Importantly, the APPJ surface activation also rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
Water contact angles were observed to be stable within a range where the conformation of biomolecules is preserved. Single and double electrode designs of APPJs were compared in their characteristics relevant to localized surface functionalization, plume length and shape. As a proof of efficacy in a biological context, APPJ treated polyethylene functionalized with fibronectin was used to demonstrate improvements in cell adhesion and proliferation.
These results have important implications for the development of a new generation of 3D bioprinters capable of spatially patterned and tailored surface functionalization performed during the 3D printing process in situ.

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 245.83

ACTaq? Hot-Start DNA Polymerase, 1000U

E3500-1000U each
EUR 535.2

ACTaq? Hot-Start DNA Polymerase, 250U

E3500-250U each
EUR 204

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

Maximo Dry-Master Mix (Lyophilized Hot-Start Polymerase)

S295H 192 rcs (20µl, in plates or tubes)
EUR 190.8

Real Time PCR mastermix 2x, (No ROX), 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400RF each
EUR 261.6

2X PCR HotStart MasterMix

G906 5.0 ml (200 Rxns)
EUR 189.6

Real-Time PCR Mastermix 2x, Regular Level of Rox, 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400 each
EUR 261.6

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

2X PCR SensTaq HotStart MasterMix

G939 5.0 ml (200 Rxns)
EUR 189.6

2X PCR HotStart MasterMix with dye

G906-dye 5.0 ml (200 Rxns)
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HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
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HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 115.68

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G009 1000 U (200 ul)
EUR 104.4

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

2X PCR SensTaq HotStart MasterMix with Dye

G939-Dye 5.0 ml (200 Rxns)
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TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

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EUR 160.8

Bloodirect DNA Polymerase (PCR)

G462 100 U (100 ul)
EUR 73.2

Bloodirect DNA Polymerase (PCR)

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EUR 104.4

Taq Plus DNA Polymerase (PCR)

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EUR 87.6

Taq Plus DNA Polymerase (PCR)

G040 1000 U (200 ul)
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Bestaq™ DNA Polymerase (PCR)

G456 250 U (50 ul)
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Bestaq™ DNA Polymerase (PCR)

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EUR 189.6

Long-Range DNA Polymerase (PCR)

G460 250 U (50 ul)
EUR 93.6

Long-Range DNA Polymerase (PCR)

G461 1000 U (200 ul)
EUR 183.6

Precision™ DNA Polymerase (PCR)

G078 500 U (100 ul)
EUR 133.2

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

HotTaq DNA Polymerase

BA00203 500U
EUR 174
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

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BA00204 1000U
EUR 286.8
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

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EUR 626.4
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

BT10201 500U
EUR 144
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

HotTaq DNA polymerase

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EUR 228
Description: High quality HotTaq polymerase for different PCR variations and downstream applications.

EZ-FD PCR High Fidelity DNA polymerase

9K-005-0019 500U
EUR 294.9

i7 Hot Start High-Fidelity DNA Polymerase - 500 Units

3283 1/EA
EUR 558

Reversible 1-min Hot Start Taq DNA Polymerase

P950 - Ask for price

Reversible 1-min Hot Start Taq DNA Polymerase

P950L - Ask for price

DNA Polymerase

ABD3082 100 ug
EUR 525.6

DNA Polymerase

ABF5235 100 ug
EUR 525.6

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 428.4

Genorise® 2 x qPCR HotStart Super Mix

GR108001 2.0 ml
EUR 199

Genorise® 2 x qPCR HotStart Master Mix

GR108002 2.0 ml
EUR 279

one-Fusion DNA Polymerase (high-speed-fidelity PCR)

S450 200 units
EUR 159.6

one-Fusion DNA Polymerase (high-speed-fidelity PCR)

S455 1000 units
EUR 393.6

HotStart Enzyme Modification Kit, 0.1 mg Reaction

29054 0.1mg
EUR 1040.4
Description: Minimum order quantity: 1 unit of 0.1mg

HotStart Enzyme Modification Kit, 0.5 mg Reaction

29054-T 0.5mg
EUR 319.2
Description: Minimum order quantity: 1 unit of 0.5mg

T4 DNA Polymerase

N101-01 2000 U
EUR 278.4

T4 DNA Polymerase

M1211-100 each
EUR 385.2

DNA polymerase ? (Rat)

10-101 20ug
EUR 465.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

DNA polymerase ? (Rat)

10-102 100ug
EUR 1107.6
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase

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EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Pfu polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

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EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: Recombinant Pfu DNA Polymerase

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9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent

Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin. Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples.
HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers.
These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids.
The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent. This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods.
The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Recombinant (E.Coli) Taq Plus DNA Polymerase

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Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)

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Taq DNA Polymerase (Mg2+ plus Buffer)

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G008 5000 U (1.0 ml)
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Taq DNA Polymerase (PCR)

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Taq DNA Polymerase (PCR)

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HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

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Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
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Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

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Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

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Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

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EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

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EUR 130.8
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Taq DNA polymerase

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EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
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Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

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Taq DNA Polymerase

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KD Plus DNA Polymerase Enzyme

abx071022-100U 100 U
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abx071022-1200U 1200 U
EUR 1078.8

KD Plus DNA Polymerase Enzyme

abx071022-200U 200 U
EUR 376.8

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq HS DNA Polymerase

P132-d1 500 U
EUR 38.28

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 71.34

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 324.8

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 567

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 159

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Recombinant Taq Dna Polymerase

27079804 EACH
EUR 117.6

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 639.6

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Human DNA Polymerase Beta Assay Kit Plus

DPB100KE 100 assays
EUR 448.28
Description: This product includes 0.800 ml of 10 x Buffer BP, 0.055 ml of 100x DNA template, 0.055 ml of 100 x dNTP mix, 0.055 ml of 100 x human DNA polymerase beta, 22 ml of Reagent U and 0.420 ml of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.

Human DNA Polymerase Gamma Assay Kit Plus

DPG100KE 100 assays
EUR 467.15
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x human DNA polymerase gamma, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit Plus

HDPA100KE1 100 assays
EUR 624.67
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP mix, 30 µl of 100 x human DNA polymerase alpha (4 µM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

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ABP-PP-ERTQ100 100U Ask for price

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Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

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EUR 145.2

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer)

P301-02 500 U
EUR 210

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2