Polymers Enables Reagent-Free Covalent

Atmospheric Pressure Plasma Jet Treatment of Polymers Enables Reagent-Free Covalent Attachment of Biomolecules for Bioprinting

3D bioprinting, where cells, hydrogels and structural polymers can be printed layer by layer into complex designs, holds great promise for advances in medicine and the biomedical sciences. In principle, this technique enables the creation of highly patient specific disease models and biomedical implants.
However, an ability to tailor surface biocompatibility and interfacial bonding between printed components, such as polymers and hydrogels, is currently lacking. Here we demonstrate that an atmospheric pressure plasma jet (APPJ) can locally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step process at desired locations.
Polyethylene and poly--caprolactone (PCL) were used as example polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removal by rigorous sodium dodecyl sulfate (SDS) washing. The immobilized protein and hydrogel layers were analyzed using Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS).
Importantly, the APPJ surface activation also rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
Water contact angles were observed to be stable within a range where the conformation of biomolecules is preserved. Single and double electrode designs of APPJs were compared in their characteristics relevant to localized surface functionalization, plume length and shape. As a proof of efficacy in a biological context, APPJ treated polyethylene functionalized with fibronectin was used to demonstrate improvements in cell adhesion and proliferation.
These results have important implications for the development of a new generation of 3D bioprinters capable of spatially patterned and tailored surface functionalization performed during the 3D printing process in situ.

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 595

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 167

HotStart Taq DNA Polymerase (5U/uL)

A4144-250U 250U
EUR 66
Description: Biotechnology

FastTaq Hotstart DNA Polymerase, 5 units/ul

T2017-001 1000 units
EUR 71

FastTaq Hotstart DNA Polymerase, 5 units/ul

T2017-005 5000 units
EUR 196

FastTaq Hotstart DNA Polymerase, 5 units/ul

T2017-010 10,000 units
EUR 360

FastTaq Hotstart DNA Polymerase, 5 units/ul

T2017-100 100,000 units
EUR 1460

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-1000Units 1000Units
EUR 320

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-100Units 100Units
EUR 145

FlashTaq HOTSTART DNA Polymerase without Buffer

MBS308007-500Units 500Units
EUR 255

3G HotStart Taq DNA Polymerase (5u/ul)

3GHST81 5x500U
EUR 896.24

HotStart Taq DNA Polymerase (Mg Plus Buffer)

MBS9141664-200Units 200Units
EUR 150

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

EZ Taq Polymerase (HotStart)

9K-005-0020 500U
EUR 245.83

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308004-1000Units 1000Units
EUR 320

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308004-100Units 100Units
EUR 145

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (MgCl2) Buffer

MBS308004-500Units 500Units
EUR 255

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308008-1000Units 1000Units
EUR 320

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308008-100Units 100Units
EUR 145

FlashTaq HOTSTART DNA Polymerase with 10X Reaction (No MgCl2) Buffer

MBS308008-500Units 500Units
EUR 255

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

ACTaq? Hot-Start DNA Polymerase, 1000U

E3500-1000U each
EUR 535.2

ACTaq? Hot-Start DNA Polymerase, 250U

E3500-250U each
EUR 204

ACTaq™ Hot-Start DNA Polymerase, 1000U

E3500 1000U
EUR 342.63

nanoTaq™ Hot-Start DNA Polymerase

DP001-0100 100 rxns500 U/100 μl
EUR 12

kemoTaq™ Hot-Start DNA Polymerase 500U

DP002-0100 100 μl
EUR 18

Antibody-Based Hot-Start DNA Polymerase

abx461028-1096tests 10 × 96 tests
EUR 2775

Antibody-Based Hot-Start DNA Polymerase

abx461028-596tests 5 × 96 tests
EUR 737.5

Antibody-Based Hot-Start DNA Polymerase

abx461028-96tests 96 tests
EUR 462.5

Aptamer-Mediated Hot-Start DNA Polymerase

abx461072-1096tests 10 × 96 tests Ask for price

Aptamer-Mediated Hot-Start DNA Polymerase

abx461072-596tests 5 × 96 tests
EUR 1700

Aptamer-Mediated Hot-Start DNA Polymerase

abx461072-96tests 96 tests
EUR 562.5

Antibody-Based Hot-Start DNA Polymerase Mix

abx461035-1096tests 10 × 96 tests Ask for price

Antibody-Based Hot-Start DNA Polymerase Mix

abx461035-596tests 5 × 96 tests
EUR 1125

Antibody-Based Hot-Start DNA Polymerase Mix

abx461035-96tests 96 tests
EUR 325

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-1096tests 10 × 96 tests
EUR 2187.5

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-596tests 5 × 96 tests
EUR 1062.5

Antibody-Based Hot-Start Taq DNA Polymerase

abx461039-96tests 96 tests
EUR 400

AlloyTaq PolymeraseTURBO, Hotstart High Fidelity with 10X Reaction Buffer

MBS308021-1000Units 1000Units
EUR 380

AlloyTaq PolymeraseTURBO, Hotstart High Fidelity with 10X Reaction Buffer

MBS308021-100Units 100Units
EUR 145

AlloyTaq PolymeraseTURBO, Hotstart High Fidelity with 10X Reaction Buffer

MBS308021-200Units 200Units
EUR 175

AlloyTaq PolymeraseTURBO, Hotstart High Fidelity with 10X Reaction Buffer

MBS308021-5000Units 5000Units
EUR 1385

AlloyTaq PolymeraseTURBO, Hotstart High Fidelity with 10X Reaction Buffer

MBS308021-500Units 500Units
EUR 255

2X PCR HotStart MasterMix

G906 5.0 ml (200 Rxns)
EUR 189.6

Real Time PCR mastermix 2x, (No ROX), 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400RF each
EUR 261.6

Maximo Dry-Master Mix (Lyophilized Hot-Start Polymerase)

S295H 192 rcs (20µl, in plates or tubes)
EUR 190.8

2X PCR SensTaq HotStart MasterMix

G939 5.0 ml (200 Rxns)
EUR 189.6

HotStart PCR Master mix (2 x)

MBS430247-250Reactions 250Reactions
EUR 235

HotStart PCR Master mix (2 x)

MBS430247-5x250Reactions 5x250Reactions
EUR 915

EntiLink™ HotStart PCR MasterMix

EQ024-1mL 1mL
EUR 10

EntiLink™ HotStart PCR MasterMix

EQ024-5mL 5mL
EUR 48

EntiLink™ HotStart PCR MasterMix

EQ024 1mL
EUR 48

2X PCR HotStart MasterMix with dye

G906-dye 5.0 ml (200 Rxns)
EUR 189.6

Hotstart PCR MasterMix (Dye), 2× Flash

W3007-1 1 ml
EUR 49

Hotstart PCR MasterMix (Dye), 2× Flash

W3007-10 10 ml
EUR 169

Real-Time PCR Mastermix 2x, Regular Level of Rox, 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400 each
EUR 261.6

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

HotStart PCR Master mix (2X, Green Dye)

BS9291 5X1mL, 5ml
EUR 251.4

HotStart PCR Master mix (2X, Green Dye)

BS9292 1ml
EUR 115.68

2X PCR SensTaq HotStart MasterMix with Dye

G939-Dye 5.0 ml (200 Rxns)
EUR 189.6

FlashTaq HOTSTART 2X Master Mix

MBS308005-1000Reactions 1000Reactions
EUR 645

FlashTaq HOTSTART 2X Master Mix

MBS308005-100Reactions 100Reactions
EUR 175

FlashTaq HOTSTART 2X Master Mix

MBS308005-250Reactions 250Reactions
EUR 250

FlashTaq HOTSTART 2X Master Mix

MBS308005-500Reactions 500Reactions
EUR 385

HotStart™ 2X Probe qPCR Master Mix

K1541-25 25ml
EUR 437.6
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix

K1541-5 5ml
EUR 123.2
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X Probe qPCR Master Mix

K1541-50 50ml
EUR 866.4
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

FlashTaq HOTSTART 2X MeanGreen Master Mix

MBS308006-1000Reactions 1000Reactions
EUR 645

FlashTaq HOTSTART 2X MeanGreen Master Mix

MBS308006-100Reactions 100Reactions
EUR 175

FlashTaq HOTSTART 2X MeanGreen Master Mix

MBS308006-250Reactions 250Reactions
EUR 195

FlashTaq HOTSTART 2X MeanGreen Master Mix

MBS308006-500Reactions 500Reactions
EUR 385

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Bloodirect DNA Polymerase (PCR)

G462 100 U (100 ul)
EUR 73.2

Bloodirect DNA Polymerase (PCR)

G463 400 U (400 ul)
EUR 104.4

HotStart™ 2X FAST Green qPCR Master Mix

K1171-25 25ml
EUR 424
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

HotStart™ 2X FAST Green qPCR Master Mix

K1171-5 5ml
EUR 132
Description: Reverse Transcription & PCR|qPCR/Real-time PCR

Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent

Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin. Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples.
HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers.
These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids.
The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent. This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods.
The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq Plus DNA Polymerase Recombinant

rAP-1063 Inquiry Ask for price

Recombinant Taq Plus DNA Polymerase

MBS142450-2500Units 2500Units
EUR 515

Recombinant Taq Plus DNA Polymerase

MBS142450-250Units 250Units
EUR 240

Recombinant Taq Plus DNA Polymerase

MBS142450-5x2500Units 5x2500Units
EUR 1990

Recombinant Taq Plus DNA Polymerase

MBS142450-750Units 750Units
EUR 310

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

TAQ Plus DNA Polymerase (2.5U/mcl)

85737 250 Units
EUR 34.98
Description: Part E

TAQ Plus DNA Polymerase (2.5U/mcl)

85737-1 1000 Units
EUR 126.32
Description: Part E

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 196.8

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-1000Units 1000Units
EUR 195

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-10KUnits 10KUnits
EUR 995

Taq DNA Polymerase (Mg Plus Buffer)

MBS9141663-5000Units 5000Units
EUR 545

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 37.29

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 290

Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20600 5000U
EUR 49

Taq DNA Polymerase (Mg2+ plus buffer)

P101-01-1000U 1,000 U
EUR 38.61

Taq DNA Polymerase (Mg2+ plus buffer)

P101-03-10000U 10,000 U
EUR 300.3

TAQ Plus DNA Polymerase (2.5U/mcl) (w/dNTP)

93561 250 Units
EUR 36.95
Description: Part E

TAQ Plus DNA Polymerase (2.5U/mcl) (w/dNTP)

93561-1 1000 Units
EUR 130.17
Description: Part E

Taq DNA Polymerase (PCR)

G008 5000 U (1.0 ml)
EUR 246

Taq DNA Polymerase (PCR)

G126 10000 U (2 x 1.0 ml)
EUR 370.8

HotStart Taq DNA Polymerase (Mg Plus Buffer)

MBS9141664-200Units 200Units
EUR 150

HotStart Taq DNA Polymerase (Mg2+ Plus Buffer)

RK20601 25 U Ask for price

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq Plus DNA Polymerse

MBS355977-3000Units 3000Units
EUR 400

Taq Plus DNA Polymerse

MBS355977-500Units 500Units
EUR 225

Taq Plus DNA Polymerse

MBS355977-5x3000Units 5x3000Units
EUR 1490

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N140 500 units
EUR 60

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N142 5x500 units
EUR 144

Maximo DFS-Plus Taq DNA Polymerase DNA-free

N144 20x500 units
EUR 440.4

hyperfusion plus DNA polymerase

K1118-100 100U
EUR 49.6
Description: Molecular Biology|Reverse Transcription & PCR

hyperfusion plus DNA polymerase

K1118-1000 1000U
EUR 200
Description: Molecular Biology|Reverse Transcription & PCR

hyperfusion plus DNA polymerase

K1118-500 500U
EUR 128
Description: Molecular Biology|Reverse Transcription & PCR

KD Plus DNA Polymerase Enzyme

abx071022-100U 100 U
EUR 292.8

KD Plus DNA Polymerase Enzyme

abx071022-1200U 1200 U
EUR 1078.8

KD Plus DNA Polymerase Enzyme

abx071022-200U 200 U
EUR 376.8

KD Plus DNA Polymerase Enzyme

abx071022-100g 100 g
EUR 300

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

E2500-01 200U
EUR 31.61
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-02 1000U
EUR 120.99
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-03 5000U
EUR 599.5
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

E2500-04 500U
EUR 69.76
Description: Ultrapure, recombinant Taq DNA polymerase for general Use (5.0 U/ul)

Taq DNA Polymerase

EK2500-01 200U
EUR 40.33
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-02 1000U
EUR 163.5
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-03 5000U
EUR 809.87
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EK2500-04 500U
EUR 90.47
Description: Taq DNA Polymerase Kit containing polymerase and dNTPs

Taq DNA Polymerase

EQ005-100ul500U 100μl/500U
EUR 15

Taq DNA Polymerase

EQ005-500ul2500U 500μl/2500U
EUR 50

Taq DNA Polymerase

G009 1000 U (200 ul)
EUR 20
Description: Taq DNA Polymerase is a highly thermostable DNA Polymerase that catalyzes the 5’-3’ synthesis of DNA. This polymerase has 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with Taq can be used with TA cloning vectors.

Taq DNA Polymerase

EQ005 100μl/500U
EUR 50

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

MBS157418-1000Units 1000Units
EUR 190

Taq DNA Polymerase

MBS157418-3000Units 3000Units
EUR 245

Taq DNA Polymerase

MBS157418-500Units 500Units
EUR 175

Taq DNA Polymerase

MBS157418-5x3000Units 5x3000Units
EUR 780

Taq DNA Polymerase

MBS402024-500Units 500Units
EUR 170

Taq DNA Polymerase

MBS402024-50Units 50Units
EUR 160

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4